PREVALENCE OF MICRONUTRIENT DEFICIENCIES IN NON PREGNANT WOMEN OF REPRODUCTIVE AGE

Authors

  • Abeera Sajid Lahore General Hospital, Pakistan. Author
  • Hafsa Hameed Thakur Alpha Clinical Developments Ltd , USA. Author https://orcid.org/0009-0007-3348-4792
  • Habib Akhtar Bahalkani Public Health Specialist, Kandhkot, Pakistan. Author
  • Aimen Ramzi GC University Faisalabad / Saint Marry College Gujranwala, Pakistan. Author
  • Gul E Khuba The University of Lahore, Pakistan. Author
  • Rameen Lutaf Ullah COMSATS University Islamabad, Sahiwal Campus, Pakistan\ Author
  • Rimsha Sattar GSK Consumer Healthcare, South Africa. Author https://orcid.org/0009-0006-6144-386X

DOI:

https://doi.org/10.71000/rjc79k63

Keywords:

Anemia, Ferritin, Folate, Micronutrient Deficiencies, Reproductive Health, Vitamin B12, Vitamin D

Abstract

Background: Micronutrient deficiencies remain a significant public health issue, particularly among women of reproductive age. Despite their importance in preconception and overall health, non-pregnant women are often overlooked in nutritional surveillance programs, especially in South Asian settings.

Objective: To determine the prevalence and patterns of iron, vitamin D, folate, and vitamin B12 deficiencies among non-pregnant women aged 15–45 years attending outpatient clinics in Lahore, Pakistan.

Methods: A cross-sectional study was conducted over six months in three outpatient clinics. A total of 360 non-pregnant women aged 15–45 years were recruited. Data collection included demographic profiling, anthropometric measurements, and biochemical assessment of serum ferritin, 25-hydroxyvitamin D, serum folate, and vitamin B12. Deficiency thresholds were based on WHO criteria. Descriptive and inferential statistics were applied using SPSS v26, and subgroup analysis was conducted by age group.

Results: The mean age of participants was 29.4 years. Vitamin D deficiency was most prevalent (52.8%), followed by iron (41.5%), folate (23.6%), and vitamin B12 (19.7%) deficiencies. The youngest age group (15–24 years) demonstrated the highest burden across all micronutrients. Mean serum levels for ferritin, vitamin D, folate, and B12 were below optimal in a substantial proportion of participants. Significant age-wise differences in serum ferritin and vitamin D levels were observed (p < 0.01).

Conclusion: A high prevalence of micronutrient deficiencies exists among non-pregnant women of reproductive age in Lahore, with younger women being most affected. These findings support the need for integrated nutritional screening and public health interventions, including food fortification and education programs, targeting this at-risk group.

Author Biographies

  • Abeera Sajid, Lahore General Hospital, Pakistan.

    PG Trainee, Lahore General Hospital, Pakistan.

  • Hafsa Hameed Thakur, Alpha Clinical Developments Ltd , USA.

    Academia : BSc ,( Hons) Biomedical Science, Kings College London. MSc, Drug Discovery and Pharma Management, University College London. Research Scientist , Alpha Clinical Developments Ltd , USA.

  • Habib Akhtar Bahalkani, Public Health Specialist, Kandhkot, Pakistan.

    Public Health Specialist, Kandhkot, Pakistan.

  • Aimen Ramzi, GC University Faisalabad / Saint Marry College Gujranwala, Pakistan.

    Lecturer, GC University Faisalabad / Saint Marry College Gujranwala, Pakistan.

  • Gul E Khuba, The University of Lahore, Pakistan.

    Nutritionist, The University of Lahore, Pakistan.

  • Rameen Lutaf Ullah, COMSATS University Islamabad, Sahiwal Campus, Pakistan\

    BS (Hons) Food Science and Nutrition, COMSATS University Islamabad, Sahiwal Campus, Pakistan. Internships: PFA, Okara DHQ, Okara, HND Clinics, Lahore, Okara, Pakistan.

  • Rimsha Sattar, GSK Consumer Healthcare, South Africa.

     Pharm-D (DUHS), EMBA- Marketing (KUBS), MSE, GSK Consumer Healthcare, South Africa.

References

Goh Y, Manger M, Duggal M, Das R, Saklani S, Agarwal S, et al. Women in Selected Communities of Punjab, India Have a High Prevalence of Iron, Zinc, Vitamin B12, and Folate Deficiencies: Implications for a Multiply-Fortified Salt Intervention. Nutrients. 2023;15.

Abdalla S, Weng Y, Mehta KM, Mahapatra T, Srikantiah S, Shah H, et al. Trends in reproductive, maternal, newborn and child health and nutrition indicators during five years of piloting and scaling-up of Ananya interventions in Bihar, India. J Glob Health. 2020;10(2):021003.

Creanga AA, Srikantiah S, Mahapatra T, Das A, Sonthalia S, Moharana PR, et al. Statewide implementation of a quality improvement initiative for reproductive, maternal, newborn and child health and nutritionin Bihar, India. J Glob Health. 2020;10(2):021008.

John S, Azizi K, Hancy A, Twin’omujuni A, Katana D, Shine J, et al. The prevalence and risk factors associated with Iron, vitamin B12 and folate deficiencies in pregnant women: A cross-sectional study in Mbeya, Tanzania. PLOS Global Public Health. 2023;3.

Vogel C, Kriznik N, Stephenson J, Barker M. Preconception nutrition: building advocacy and social movements to stimulate action. J Dev Orig Health Dis. 2021;12(1):141-6.

McHau G, Masanja H, Killel E, Azizi K, Lukindo T, Hancy A, et al. Micronutrient deficiencies and their co-occurrence among pregnant women in Mbeya region, Tanzania. PLOS ONE. 2024;19.

Neufeld L, Sethi V, Woldeyahannes M, McDonald C, Beal T, Petry N, et al. Micronutrient deficiencies among preschool-aged children and women of reproductive age worldwide: a pooled analysis of individual-level data from population-representative surveys. The Lancet Global health. 2022;10.

Chong M, Bui C, Jaisamrarn U, Pacquing-Songco D, Shaw S, Tam C, et al. A landscape of micronutrient status in women through the reproductive years: Insights from seven regions in Asia. Women's Health. 2020;16.

Ahmed F, Ahmad G, Brand T, Zeeb H. Key indicators for appraising adolescent sexual and reproductive health in South Asia: international expert consensus exercise using the Delphi technique. Glob Health Action. 2020;13(1):1830555.

Darmstadt GL, Weng Y, Pepper KT, Ward VC, Mehta KM, Borkum E, et al. Impact of the Ananya program on reproductive, maternal, newborn and child health and nutrition in Bihar, India: early results from a quasi-experimental study. J Glob Health. 2020;10(2):021002.

Ward VC, Raheel H, Weng Y, Mehta KM, Dutt P, Mitra R, et al. Impact of mHealth interventions for reproductive, maternal, newborn and child health and nutrition at scale: BBC Media Action and the Ananya program in Bihar, India. J Glob Health. 2020;10(2):021005.

Mehta KM, Irani L, Chaudhuri I, Mahapatra T, Schooley J, Srikantiah S, et al. Health layering of self-help groups: impacts on reproductive, maternal, newborn and child health and nutrition in Bihar, India. J Glob Health. 2020;10(2):021007.

Mehta KM, Irani L, Chaudhuri I, Mahapatra T, Schooley J, Srikantiah S, et al. Health impact of self-help groups scaled-up statewide in Bihar, India. J Glob Health. 2020;10(2):021006.

Abdalla S, Pair E, Mehta KM, Ward VC, Darmstadt GL. Geospatial variations in trends of reproductive, maternal, newborn and child health and nutrition indicators at block level in Bihar, India, during scale-up of Ananya program interventions. J Glob Health. 2020;10(2):021004.

Ngassa N, Masumo R, Hancy A, Kabula E, Killel E, Nusurupia J, et al. Food and nutrient intake among non-pregnant, non-lactating women of reproductive age of Mbeya in Tanzania: A repeated 24-hour dietary recall. PLOS Global Public Health. 2024;4.

Ahmed KY, Rwabilimbo AG, Abrha S, Page A, Arora A, Tadese F, et al. Factors associated with underweight, overweight, and obesity in reproductive age Tanzanian women. PLoS One. 2020;15(8):e0237720.

Storto ME, Bailey-Straebler SM, Susser LC. Exploring the Relationship Between Eating Disorders and Reproductive Health, with a Focus on Fertility, Obstetric, and Fetal Outcomes: A Narrative Review. Curr Nutr Rep. 2025;14(1):51.

Ward VC, Weng Y, Bentley J, Carmichael SL, Mehta KM, Mahmood W, et al. Evaluation of a large-scale reproductive, maternal, newborn and child health and nutrition program in Bihar, India, through an equity lens. J Glob Health. 2020;10(2):021011.

Ahmad F, Calabrese CM, Terranegra A. The Era of Precision Nutrition in the Field of Reproductive Health and Pregnancy. Nutrients. 2023;15(14).

Habibi N, Livingstone KM, Edwards S, Grieger JA. Do Older Women of Reproductive Age Have Better Diet Quality than Younger Women of Reproductive Age? Nutrients. 2021;13(11).

Dass M, Nyako J, Tortoe C, Fanou-Fogny N, Nago E, Hounhouigan J, et al. Comparison of Micronutrient Intervention Strategies in Ghana and Benin to Cover Micronutrient Needs: Simulation of Bene-Fits and Risks in Women of Reproductive Age. Nutrients. 2021;13(7).

Yıldızdaş H, Erdogan BG, Tepe T, Görüroglu O, Ozlü F, Satar M, et al. Birth weight, head circumference, and length of newborns are unaffected by maternal levels of vitamin B12 and folate. Nutrition research. 2022;104:101-7.

Nazir I, Wadood M, Nazir S, Rizwan M, Ayub MA, Nasir SS. Assessment of RBC Folate and Serum Vitamin B12 Levels in Pakistani Women of Reproductive Age Group: An Approach towards Prevention of Adverse Pregnancy Outcomes. Journal of Pharmaceutical Research International. 2022.

Ross DA. Adolescent health in sub-Saharan Africa: results from the ARISE Network. Trop Med Int Health. 2021;26(4):386-7.

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Published

2025-08-06

How to Cite

1.
Sajid A, Hafsa Hameed Thakur, Habib Akhtar Bahalkani, Aimen Ramzi, Gul E Khuba, Rameen Lutaf Ullah, et al. PREVALENCE OF MICRONUTRIENT DEFICIENCIES IN NON PREGNANT WOMEN OF REPRODUCTIVE AGE. IJHR [Internet]. 2025 Aug. 6 [cited 2025 Aug. 28];3(4 (Health and Rehabilitation):436-42. Available from: https://insightsjhr.com/index.php/home/article/view/1202